β1 integrin aiib2  (Developmental Studies Hybridoma Bank)

Journal: bioRxiv

Article Title: Activating FGFR1 restores Integrin-β1–mediated fibronectin sensing in satellite cells of aged mice

doi: 10.64898/2026.02.18.706475

Figure Lengend Snippet: (A) Experimental scheme for treatment of young caFGFR1– mice with tamoxifen and doxycycline chow, followed by myofiber isolation and culture prior to fixation. (B) Representative images of myofibers cultured with or without FGF-2, in the presence of AIIB2 or TS2/16, where SCs were identified by Syndecan-4 immunoreactivity. Scale bars, 100 μm. (C) Number of Syndecan-4 + cells on myofibers cultured with or without FGF-2, in the presence of AIIB2 or TS2/16. n > 10 myofibers analyzed with samples from 3 mice, and statistics performed based on 3 independent mice; data are presented as mean ± SD; **P < 0.01 and ***P < 0.001 in a two-way ANOVA test. (D) Representative images of SCs on myofibers cultured with or without FGF-2, in the presence of AIIB2 or TS2/16, identified by Syndecan-4 immunoreactivity and assessed for phospho-histone H3 (pHH3) and PARD3 immunoreactivity. Scale bars, 10 μm. (E and F) Percentage of pHH3 + immunoreactive cells (E) and percentage of asymmetric division (F) on myofibers cultured with or without FGF-2, in the presence of AIIB2 or TS2/16. n > 200 Syndecan-4 + cells analyzed with samples from 3 mice, and statistics performed based on 3 independent mice; data are presented as mean ± SD; *P < 0.05 and ***P < 0.001 in a two-way ANOVA test.

Article Snippet: When indicated, myofibers were either fixed in 4% paraformaldehyde for 10 min immediately after isolation or cultured in suspension at 37 °C and 5% CO2 for 36 h prior to fixation or 24 h prior to encapsulation, with supplementation of 0.5 nM fibroblast growth factor 2, 1 μg/mL AIIB2 (Developmental Studies Hybridoma Bank), or 1 μg/mL TS2/16 (eBioscience) as specified.

Techniques: Isolation, Cell Culture

Journal: bioRxiv

Article Title: Activating FGFR1 restores Integrin-β1–mediated fibronectin sensing in satellite cells of aged mice

doi: 10.64898/2026.02.18.706475

Figure Lengend Snippet: (A) Experimental scheme showing treatment of aged caFGFR1– and caFGFR1+ mice and the timeline for myofiber harvest and culture. (B) Representative images of myofibers from aged caFGFR1–and caFGFR1+ mice cultured in the presence of AIIB2 antibody or TS2/16 antibody, where SCs were identified by Syndecan-4 immunoreactivity. Scale bars, 100 μm. (C) Syndecan-4 + cells on myofibers quantified from aged caFGFR1– and caFGFR1+ mice cultured in the presence of AIIB2 or TS2/16. n > 10 myofibers analyzed with samples from 3 mice, and statistics performed based on 3 independent mice; data are presented as mean ± SD; *P < 0.05 and ***P < 0.001 in a two-way ANOVA test. (D) Representative images of SCs on myofibers from aged caFGFR1– and caFGFR1+ mice cultured in the presence of AIIB2 antibody or TS2/16 antibody, where SCs were identified by Syndecan-4 immunoreactivity and assayed for pHH3 immunoreactivity and PARD3 immunoreactivity. Scale bars, 10 μm. (E and F) The percentage of pHH3 + cells (E) and percentage of asymmetric division (F) on myofibers from aged caFGFR1– and caFGFR1+ mice cultured in the presence of AIIB2 or TS2/16. n > 200 Syndecan-4 + cells analyzed with samples from 3 mice, and statistics performed based on 3 independent mice; data are presented as mean ± SD; *P < 0.05, **P < 0.01, and ***P < 0.001 in a two-way ANOVA test.

Article Snippet: When indicated, myofibers were either fixed in 4% paraformaldehyde for 10 min immediately after isolation or cultured in suspension at 37 °C and 5% CO2 for 36 h prior to fixation or 24 h prior to encapsulation, with supplementation of 0.5 nM fibroblast growth factor 2, 1 μg/mL AIIB2 (Developmental Studies Hybridoma Bank), or 1 μg/mL TS2/16 (eBioscience) as specified.

Techniques: Cell Culture

Journal: bioRxiv

Article Title: Nascent extracellular matrix converts biomaterial cues into cell fate decisions

doi: 10.64898/2026.01.10.698826

Figure Lengend Snippet: a. Timeline and schematic showing the blocking of cell adhesion to nECM using an integrin β1 (ITGB1) function perturbing antibody for 1 hour pre-embedding and for 7 consecutive days . b. Representative fluorescent images of the incorporation of EdU in chondrocytes cultured without (Ctrl) and with ITGB1 inhibition (ITGB1i) in high and low modification hydrogels at day 7 (scale bar = 100 μm). c. Quantification of the incorporation of EdU in chondrocytes cultured without (Ctrl) and with ITGB1 inhibition (ITGB1i) in high and low modification hydrogels at day 7 (low-Ctrl: n = 23 ROIs, N = 3; low-ITGB1i: n = 30 ROIs, N = 3; high-Ctrl: n = 23 ROIs, N = 3; low-ITGB1i: n = 27 ROIs, N = 3). d. Representative fluorescent images of Sox9 immunofluorescence (dashed line outlines nuclei) of chondrocytes cultured without (Ctrl) and with ITGB1 inhibition (ITGB1i) in low and high modification hydrogels at day 7 (scale bar = 10 μm). e. Quantification of Sox9 nucleus-to-cytoplasm (NC) ratio of chondrocytes cultured without (Ctrl) and with ITGB1 inhibition (ITGB1i) in low and high modification hydrogels at day 7 (low-Ctrl: n = 138 cells, N = 4; low-ITGB1i: n = 133 cells, N = 4; high-Ctrl: n = 124 cells, N = 4; high-ITGBi: n = 138 cells, N = 4). a-e. N = number of independent experiments ****p < 0.0001, *p < 0.05, error bar = standard deviation, ns: no significant difference by two-way ANOVA with Tukey’s multiple comparisons test.

Article Snippet: For perturbation studies, media was supplemented with either an anti-CD44 antibody (DSHB, H4C4, 2.5 μg/mL, day 0-3) or anti-integrin β1 antibody (anti-ITGB1, DSHB, AIIB2, 2.5 μg/mL, day 0-7).

Techniques: Blocking Assay, Cell Culture, Inhibition, Modification, Immunofluorescence, Standard Deviation